Analysis Note

ControlRat E18 cortex cells

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Anti-NeuN (rabbit) Antibody, Cy3 Conjugate is an antibody against NeuN (rabbit) for use in IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Immunogen

GST-tagged recombinant protein corresponding to mouse NeuN.

Epitope: N-terminus

Physical form

Affinity purified

Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunocytochemistry in rat E18 cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex cells.

Specificity

The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.

This antibody recognizes NeuN at the N-terminus.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates.